MTT Assay:Article Title: Oxaliplatin resistance in colorectal cancer enhances TRAIL sensitivity via death receptor 4 upregulation and lipid raft localization
Article Snippet: .. Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Homo sapiens) SW620 adenocarcinoma, colorectal, Dukes’ type C ATCC #CCL-227 RRID:CVCL_0547 L15 Media Cell line (Homo sapiens) SW480 adenocarcinoma, colorectal, Dukes’ type B ATCC #CCL-228 RRID:CVCL_0546 L15 Media Cell line (Homo sapiens) HT29 adenocarcinoma, colorectal ATCC #HTB-38 RRID:CVCL_0320 McCoy’s 5A Media Cell line (Homo sapiens) HCT116 carcinoma, colorectal ATCC #CCL-247 RRID:CVCL_0291 McCoy’s 5A Media Cell line (Homo sapiens) SW620 OxR adenocarcinoma, colorectal, Dukes’ type C Kobe Pharmaceutical University #CCL-227 RRID:CVCL_4V77 L15 Media Cell line (Homo sapiens) SW480 OxR adenocarcinoma, colorectal, Dukes’ type B MD Anderson Cancer Center Characterized Cell Line Core #CCL-228 RRID:CVCL_AU18 L15 Media Cell line (Homo sapiens) HT29 OxR adenocarcinoma, colorectal MD Anderson Cancer Center Characterized Cell Line Core #HTB-38 RRID:CVCL_ 5949 McCoy’s 5A Media Cell line (Homo sapiens) HCT116 OxR carcinoma, colorectal MD Anderson Cancer Center Characterized Cell Line Core #CCL-247 RRID:CVCL_4V73 McCoy’s 5A Media Chemical compound, drug Oxaliplatin MedChemExpress Cat# HY-17371 CAS No: 61825-94-3 Commercial assay or kit MTT Assay Kit Abcam Cat# ab211091 Peptide, recombinant protein Recombinant Human s TRAIL/Apo2L PeproTech Cat# 310-04 Antibody Mouse AntiTNFRSF10A Recombinant Antibody (clone mAY4) Creative Biolabs Cat# HPAB-1616-FY Mapatumumab (0.01–10 mg/ml) Commercial assay or kit FITC Annexin-V Apoptosis Detection Kit I BD Pharmingen Cat# 556547 Includes propidium iodide Software, algorithm FlowJo v10.7.1 FlowJo RRID:SCR_008520 Commercial assay or kit JC1 – Mitochondrial Membrane Potential Assay Kit Abcam Cat# ab113850 Commercial assay or kit RNeasy Plus Mini Kit Qiagen Cat# 74134 Commercial assay or kit RT2 First Strand Kit Qiagen Cat# 330404 Commercial assay or kit RT2 Profiler PCR Human Apoptosis Array Qiagen Cat# PAHS-012Z Continued on next page Greenlee et al. eLife 2021;10:e67750. .. DOI: https://doi.org/10.7554/eLife.67750 19 of 30 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm CFX Maestro Software Bio-Rad RRID:SCR_018064 Software, algorithm GeneGlobe Data Analysis Center Qiagen RRID:SCR_021211 Commercial assay or kit Gene Knockout Kit v2 – Human CASP10 with Cas9 2NLS Nuclease Synthego sgRNA:Cas9 (90 pmol:10 pmol) Commercial assay or kit Vybrant Alexa Fluor 488 Lipid Raft Labeling Kit Invitrogen Cat# V34403 Commercial assay or kit Vybrant Alexa Fluor 555 Lipid Raft Labeling Kit Invitrogen Cat# V34404 Antibody Mouse anti-human CD261 (DR4) Monoclonal Antibody (clone DJR1) Invitrogen Cat# 14-6644-82 RRID:AB_468188 (1:50 IF) Antibody Mouse anti-human CD262 (DR5) Monoclonal Antibody (clone DJR2-4) Invitrogen Cat# 14-9908-82 RRID:AB_468592 (1:50 IF) Antibody Alexa Fluor 555 goat anti-mouse IgG (H+L) Invitrogen Cat# A28180 RRID:AB_2536164 (1:1000 IF) Other DAPI Invitrogen Cat# D1306 RRID:AB_2629482 (1 mg/ml) Software, algorithm Fiji – ImageJ FIJI RRID:SCR_002285 JaCOP plugin Antibody Human TruStain FcX BioLegend Cat# 422301 RRID:AB_2818986 Antibody PE mouse antihuman CD261 (DR4) (clone DJR1) BioLegend Cat# 307206 RRID:AB_2287472 (5 ml per sample, FC) Antibody PE mouse antihuman CD262 (DR5) (clone DJR2-4) BioLegend Cat# 307406 RRID:AB_2204926 (5 ml per sample, FC) Antibody PE mouse antihuman TRAILR3 (DcR1) (clone DJR3) BioLegend Cat# 307006 RRID:AB_2205089 (5 ml per sample, FC) Antibody PE mouse antihuman TRAILR4 (DcR2) (clone 104918) BioLegend Cat# FAB633P RRID:AB_2205217 (5 ml per sample, FC) Antibody PE Mouse IgG1 k Isotype Control (clone MOPC-21) BioLegend Cat# 400114 RRID:AB_326435 (5 ml per sample, FC) Antibody FITC mouse anti-human DR4 (clone DR-4-02) Thermo Fisher Scientific Cat# MA1-19757 RRID:AB_1955203 (5 ml per sample, FC) Chemical compound, drug Resveratrol Sigma-Aldrich Cat# R5010-100MG RRID:AB_309682 CAS: 501-36-0 Continued on next page Greenlee et al. eLife 2021;10:e67750.
Recombinant:Article Title: Oxaliplatin resistance in colorectal cancer enhances TRAIL sensitivity via death receptor 4 upregulation and lipid raft localization
Article Snippet: .. Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Homo sapiens) SW620 adenocarcinoma, colorectal, Dukes’ type C ATCC #CCL-227 RRID:CVCL_0547 L15 Media Cell line (Homo sapiens) SW480 adenocarcinoma, colorectal, Dukes’ type B ATCC #CCL-228 RRID:CVCL_0546 L15 Media Cell line (Homo sapiens) HT29 adenocarcinoma, colorectal ATCC #HTB-38 RRID:CVCL_0320 McCoy’s 5A Media Cell line (Homo sapiens) HCT116 carcinoma, colorectal ATCC #CCL-247 RRID:CVCL_0291 McCoy’s 5A Media Cell line (Homo sapiens) SW620 OxR adenocarcinoma, colorectal, Dukes’ type C Kobe Pharmaceutical University #CCL-227 RRID:CVCL_4V77 L15 Media Cell line (Homo sapiens) SW480 OxR adenocarcinoma, colorectal, Dukes’ type B MD Anderson Cancer Center Characterized Cell Line Core #CCL-228 RRID:CVCL_AU18 L15 Media Cell line (Homo sapiens) HT29 OxR adenocarcinoma, colorectal MD Anderson Cancer Center Characterized Cell Line Core #HTB-38 RRID:CVCL_ 5949 McCoy’s 5A Media Cell line (Homo sapiens) HCT116 OxR carcinoma, colorectal MD Anderson Cancer Center Characterized Cell Line Core #CCL-247 RRID:CVCL_4V73 McCoy’s 5A Media Chemical compound, drug Oxaliplatin MedChemExpress Cat# HY-17371 CAS No: 61825-94-3 Commercial assay or kit MTT Assay Kit Abcam Cat# ab211091 Peptide, recombinant protein Recombinant Human s TRAIL/Apo2L PeproTech Cat# 310-04 Antibody Mouse AntiTNFRSF10A Recombinant Antibody (clone mAY4) Creative Biolabs Cat# HPAB-1616-FY Mapatumumab (0.01–10 mg/ml) Commercial assay or kit FITC Annexin-V Apoptosis Detection Kit I BD Pharmingen Cat# 556547 Includes propidium iodide Software, algorithm FlowJo v10.7.1 FlowJo RRID:SCR_008520 Commercial assay or kit JC1 – Mitochondrial Membrane Potential Assay Kit Abcam Cat# ab113850 Commercial assay or kit RNeasy Plus Mini Kit Qiagen Cat# 74134 Commercial assay or kit RT2 First Strand Kit Qiagen Cat# 330404 Commercial assay or kit RT2 Profiler PCR Human Apoptosis Array Qiagen Cat# PAHS-012Z Continued on next page Greenlee et al. eLife 2021;10:e67750. .. DOI: https://doi.org/10.7554/eLife.67750 19 of 30 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm CFX Maestro Software Bio-Rad RRID:SCR_018064 Software, algorithm GeneGlobe Data Analysis Center Qiagen RRID:SCR_021211 Commercial assay or kit Gene Knockout Kit v2 – Human CASP10 with Cas9 2NLS Nuclease Synthego sgRNA:Cas9 (90 pmol:10 pmol) Commercial assay or kit Vybrant Alexa Fluor 488 Lipid Raft Labeling Kit Invitrogen Cat# V34403 Commercial assay or kit Vybrant Alexa Fluor 555 Lipid Raft Labeling Kit Invitrogen Cat# V34404 Antibody Mouse anti-human CD261 (DR4) Monoclonal Antibody (clone DJR1) Invitrogen Cat# 14-6644-82 RRID:AB_468188 (1:50 IF) Antibody Mouse anti-human CD262 (DR5) Monoclonal Antibody (clone DJR2-4) Invitrogen Cat# 14-9908-82 RRID:AB_468592 (1:50 IF) Antibody Alexa Fluor 555 goat anti-mouse IgG (H+L) Invitrogen Cat# A28180 RRID:AB_2536164 (1:1000 IF) Other DAPI Invitrogen Cat# D1306 RRID:AB_2629482 (1 mg/ml) Software, algorithm Fiji – ImageJ FIJI RRID:SCR_002285 JaCOP plugin Antibody Human TruStain FcX BioLegend Cat# 422301 RRID:AB_2818986 Antibody PE mouse antihuman CD261 (DR4) (clone DJR1) BioLegend Cat# 307206 RRID:AB_2287472 (5 ml per sample, FC) Antibody PE mouse antihuman CD262 (DR5) (clone DJR2-4) BioLegend Cat# 307406 RRID:AB_2204926 (5 ml per sample, FC) Antibody PE mouse antihuman TRAILR3 (DcR1) (clone DJR3) BioLegend Cat# 307006 RRID:AB_2205089 (5 ml per sample, FC) Antibody PE mouse antihuman TRAILR4 (DcR2) (clone 104918) BioLegend Cat# FAB633P RRID:AB_2205217 (5 ml per sample, FC) Antibody PE Mouse IgG1 k Isotype Control (clone MOPC-21) BioLegend Cat# 400114 RRID:AB_326435 (5 ml per sample, FC) Antibody FITC mouse anti-human DR4 (clone DR-4-02) Thermo Fisher Scientific Cat# MA1-19757 RRID:AB_1955203 (5 ml per sample, FC) Chemical compound, drug Resveratrol Sigma-Aldrich Cat# R5010-100MG RRID:AB_309682 CAS: 501-36-0 Continued on next page Greenlee et al. eLife 2021;10:e67750.
Software:Article Title: Oxaliplatin resistance in colorectal cancer enhances TRAIL sensitivity via death receptor 4 upregulation and lipid raft localization
Article Snippet: .. Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Homo sapiens) SW620 adenocarcinoma, colorectal, Dukes’ type C ATCC #CCL-227 RRID:CVCL_0547 L15 Media Cell line (Homo sapiens) SW480 adenocarcinoma, colorectal, Dukes’ type B ATCC #CCL-228 RRID:CVCL_0546 L15 Media Cell line (Homo sapiens) HT29 adenocarcinoma, colorectal ATCC #HTB-38 RRID:CVCL_0320 McCoy’s 5A Media Cell line (Homo sapiens) HCT116 carcinoma, colorectal ATCC #CCL-247 RRID:CVCL_0291 McCoy’s 5A Media Cell line (Homo sapiens) SW620 OxR adenocarcinoma, colorectal, Dukes’ type C Kobe Pharmaceutical University #CCL-227 RRID:CVCL_4V77 L15 Media Cell line (Homo sapiens) SW480 OxR adenocarcinoma, colorectal, Dukes’ type B MD Anderson Cancer Center Characterized Cell Line Core #CCL-228 RRID:CVCL_AU18 L15 Media Cell line (Homo sapiens) HT29 OxR adenocarcinoma, colorectal MD Anderson Cancer Center Characterized Cell Line Core #HTB-38 RRID:CVCL_ 5949 McCoy’s 5A Media Cell line (Homo sapiens) HCT116 OxR carcinoma, colorectal MD Anderson Cancer Center Characterized Cell Line Core #CCL-247 RRID:CVCL_4V73 McCoy’s 5A Media Chemical compound, drug Oxaliplatin MedChemExpress Cat# HY-17371 CAS No: 61825-94-3 Commercial assay or kit MTT Assay Kit Abcam Cat# ab211091 Peptide, recombinant protein Recombinant Human s TRAIL/Apo2L PeproTech Cat# 310-04 Antibody Mouse AntiTNFRSF10A Recombinant Antibody (clone mAY4) Creative Biolabs Cat# HPAB-1616-FY Mapatumumab (0.01–10 mg/ml) Commercial assay or kit FITC Annexin-V Apoptosis Detection Kit I BD Pharmingen Cat# 556547 Includes propidium iodide Software, algorithm FlowJo v10.7.1 FlowJo RRID:SCR_008520 Commercial assay or kit JC1 – Mitochondrial Membrane Potential Assay Kit Abcam Cat# ab113850 Commercial assay or kit RNeasy Plus Mini Kit Qiagen Cat# 74134 Commercial assay or kit RT2 First Strand Kit Qiagen Cat# 330404 Commercial assay or kit RT2 Profiler PCR Human Apoptosis Array Qiagen Cat# PAHS-012Z Continued on next page Greenlee et al. eLife 2021;10:e67750. .. DOI: https://doi.org/10.7554/eLife.67750 19 of 30 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm CFX Maestro Software Bio-Rad RRID:SCR_018064 Software, algorithm GeneGlobe Data Analysis Center Qiagen RRID:SCR_021211 Commercial assay or kit Gene Knockout Kit v2 – Human CASP10 with Cas9 2NLS Nuclease Synthego sgRNA:Cas9 (90 pmol:10 pmol) Commercial assay or kit Vybrant Alexa Fluor 488 Lipid Raft Labeling Kit Invitrogen Cat# V34403 Commercial assay or kit Vybrant Alexa Fluor 555 Lipid Raft Labeling Kit Invitrogen Cat# V34404 Antibody Mouse anti-human CD261 (DR4) Monoclonal Antibody (clone DJR1) Invitrogen Cat# 14-6644-82 RRID:AB_468188 (1:50 IF) Antibody Mouse anti-human CD262 (DR5) Monoclonal Antibody (clone DJR2-4) Invitrogen Cat# 14-9908-82 RRID:AB_468592 (1:50 IF) Antibody Alexa Fluor 555 goat anti-mouse IgG (H+L) Invitrogen Cat# A28180 RRID:AB_2536164 (1:1000 IF) Other DAPI Invitrogen Cat# D1306 RRID:AB_2629482 (1 mg/ml) Software, algorithm Fiji – ImageJ FIJI RRID:SCR_002285 JaCOP plugin Antibody Human TruStain FcX BioLegend Cat# 422301 RRID:AB_2818986 Antibody PE mouse antihuman CD261 (DR4) (clone DJR1) BioLegend Cat# 307206 RRID:AB_2287472 (5 ml per sample, FC) Antibody PE mouse antihuman CD262 (DR5) (clone DJR2-4) BioLegend Cat# 307406 RRID:AB_2204926 (5 ml per sample, FC) Antibody PE mouse antihuman TRAILR3 (DcR1) (clone DJR3) BioLegend Cat# 307006 RRID:AB_2205089 (5 ml per sample, FC) Antibody PE mouse antihuman TRAILR4 (DcR2) (clone 104918) BioLegend Cat# FAB633P RRID:AB_2205217 (5 ml per sample, FC) Antibody PE Mouse IgG1 k Isotype Control (clone MOPC-21) BioLegend Cat# 400114 RRID:AB_326435 (5 ml per sample, FC) Antibody FITC mouse anti-human DR4 (clone DR-4-02) Thermo Fisher Scientific Cat# MA1-19757 RRID:AB_1955203 (5 ml per sample, FC) Chemical compound, drug Resveratrol Sigma-Aldrich Cat# R5010-100MG RRID:AB_309682 CAS: 501-36-0 Continued on next page Greenlee et al. eLife 2021;10:e67750.
Membrane:Article Title: Oxaliplatin resistance in colorectal cancer enhances TRAIL sensitivity via death receptor 4 upregulation and lipid raft localization
Article Snippet: .. Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Homo sapiens) SW620 adenocarcinoma, colorectal, Dukes’ type C ATCC #CCL-227 RRID:CVCL_0547 L15 Media Cell line (Homo sapiens) SW480 adenocarcinoma, colorectal, Dukes’ type B ATCC #CCL-228 RRID:CVCL_0546 L15 Media Cell line (Homo sapiens) HT29 adenocarcinoma, colorectal ATCC #HTB-38 RRID:CVCL_0320 McCoy’s 5A Media Cell line (Homo sapiens) HCT116 carcinoma, colorectal ATCC #CCL-247 RRID:CVCL_0291 McCoy’s 5A Media Cell line (Homo sapiens) SW620 OxR adenocarcinoma, colorectal, Dukes’ type C Kobe Pharmaceutical University #CCL-227 RRID:CVCL_4V77 L15 Media Cell line (Homo sapiens) SW480 OxR adenocarcinoma, colorectal, Dukes’ type B MD Anderson Cancer Center Characterized Cell Line Core #CCL-228 RRID:CVCL_AU18 L15 Media Cell line (Homo sapiens) HT29 OxR adenocarcinoma, colorectal MD Anderson Cancer Center Characterized Cell Line Core #HTB-38 RRID:CVCL_ 5949 McCoy’s 5A Media Cell line (Homo sapiens) HCT116 OxR carcinoma, colorectal MD Anderson Cancer Center Characterized Cell Line Core #CCL-247 RRID:CVCL_4V73 McCoy’s 5A Media Chemical compound, drug Oxaliplatin MedChemExpress Cat# HY-17371 CAS No: 61825-94-3 Commercial assay or kit MTT Assay Kit Abcam Cat# ab211091 Peptide, recombinant protein Recombinant Human s TRAIL/Apo2L PeproTech Cat# 310-04 Antibody Mouse AntiTNFRSF10A Recombinant Antibody (clone mAY4) Creative Biolabs Cat# HPAB-1616-FY Mapatumumab (0.01–10 mg/ml) Commercial assay or kit FITC Annexin-V Apoptosis Detection Kit I BD Pharmingen Cat# 556547 Includes propidium iodide Software, algorithm FlowJo v10.7.1 FlowJo RRID:SCR_008520 Commercial assay or kit JC1 – Mitochondrial Membrane Potential Assay Kit Abcam Cat# ab113850 Commercial assay or kit RNeasy Plus Mini Kit Qiagen Cat# 74134 Commercial assay or kit RT2 First Strand Kit Qiagen Cat# 330404 Commercial assay or kit RT2 Profiler PCR Human Apoptosis Array Qiagen Cat# PAHS-012Z Continued on next page Greenlee et al. eLife 2021;10:e67750. .. DOI: https://doi.org/10.7554/eLife.67750 19 of 30 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm CFX Maestro Software Bio-Rad RRID:SCR_018064 Software, algorithm GeneGlobe Data Analysis Center Qiagen RRID:SCR_021211 Commercial assay or kit Gene Knockout Kit v2 – Human CASP10 with Cas9 2NLS Nuclease Synthego sgRNA:Cas9 (90 pmol:10 pmol) Commercial assay or kit Vybrant Alexa Fluor 488 Lipid Raft Labeling Kit Invitrogen Cat# V34403 Commercial assay or kit Vybrant Alexa Fluor 555 Lipid Raft Labeling Kit Invitrogen Cat# V34404 Antibody Mouse anti-human CD261 (DR4) Monoclonal Antibody (clone DJR1) Invitrogen Cat# 14-6644-82 RRID:AB_468188 (1:50 IF) Antibody Mouse anti-human CD262 (DR5) Monoclonal Antibody (clone DJR2-4) Invitrogen Cat# 14-9908-82 RRID:AB_468592 (1:50 IF) Antibody Alexa Fluor 555 goat anti-mouse IgG (H+L) Invitrogen Cat# A28180 RRID:AB_2536164 (1:1000 IF) Other DAPI Invitrogen Cat# D1306 RRID:AB_2629482 (1 mg/ml) Software, algorithm Fiji – ImageJ FIJI RRID:SCR_002285 JaCOP plugin Antibody Human TruStain FcX BioLegend Cat# 422301 RRID:AB_2818986 Antibody PE mouse antihuman CD261 (DR4) (clone DJR1) BioLegend Cat# 307206 RRID:AB_2287472 (5 ml per sample, FC) Antibody PE mouse antihuman CD262 (DR5) (clone DJR2-4) BioLegend Cat# 307406 RRID:AB_2204926 (5 ml per sample, FC) Antibody PE mouse antihuman TRAILR3 (DcR1) (clone DJR3) BioLegend Cat# 307006 RRID:AB_2205089 (5 ml per sample, FC) Antibody PE mouse antihuman TRAILR4 (DcR2) (clone 104918) BioLegend Cat# FAB633P RRID:AB_2205217 (5 ml per sample, FC) Antibody PE Mouse IgG1 k Isotype Control (clone MOPC-21) BioLegend Cat# 400114 RRID:AB_326435 (5 ml per sample, FC) Antibody FITC mouse anti-human DR4 (clone DR-4-02) Thermo Fisher Scientific Cat# MA1-19757 RRID:AB_1955203 (5 ml per sample, FC) Chemical compound, drug Resveratrol Sigma-Aldrich Cat# R5010-100MG RRID:AB_309682 CAS: 501-36-0 Continued on next page Greenlee et al. eLife 2021;10:e67750.
Polymerase Chain Reaction:Article Title: Oxaliplatin resistance in colorectal cancer enhances TRAIL sensitivity via death receptor 4 upregulation and lipid raft localization
Article Snippet: .. Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Homo sapiens) SW620 adenocarcinoma, colorectal, Dukes’ type C ATCC #CCL-227 RRID:CVCL_0547 L15 Media Cell line (Homo sapiens) SW480 adenocarcinoma, colorectal, Dukes’ type B ATCC #CCL-228 RRID:CVCL_0546 L15 Media Cell line (Homo sapiens) HT29 adenocarcinoma, colorectal ATCC #HTB-38 RRID:CVCL_0320 McCoy’s 5A Media Cell line (Homo sapiens) HCT116 carcinoma, colorectal ATCC #CCL-247 RRID:CVCL_0291 McCoy’s 5A Media Cell line (Homo sapiens) SW620 OxR adenocarcinoma, colorectal, Dukes’ type C Kobe Pharmaceutical University #CCL-227 RRID:CVCL_4V77 L15 Media Cell line (Homo sapiens) SW480 OxR adenocarcinoma, colorectal, Dukes’ type B MD Anderson Cancer Center Characterized Cell Line Core #CCL-228 RRID:CVCL_AU18 L15 Media Cell line (Homo sapiens) HT29 OxR adenocarcinoma, colorectal MD Anderson Cancer Center Characterized Cell Line Core #HTB-38 RRID:CVCL_ 5949 McCoy’s 5A Media Cell line (Homo sapiens) HCT116 OxR carcinoma, colorectal MD Anderson Cancer Center Characterized Cell Line Core #CCL-247 RRID:CVCL_4V73 McCoy’s 5A Media Chemical compound, drug Oxaliplatin MedChemExpress Cat# HY-17371 CAS No: 61825-94-3 Commercial assay or kit MTT Assay Kit Abcam Cat# ab211091 Peptide, recombinant protein Recombinant Human s TRAIL/Apo2L PeproTech Cat# 310-04 Antibody Mouse AntiTNFRSF10A Recombinant Antibody (clone mAY4) Creative Biolabs Cat# HPAB-1616-FY Mapatumumab (0.01–10 mg/ml) Commercial assay or kit FITC Annexin-V Apoptosis Detection Kit I BD Pharmingen Cat# 556547 Includes propidium iodide Software, algorithm FlowJo v10.7.1 FlowJo RRID:SCR_008520 Commercial assay or kit JC1 – Mitochondrial Membrane Potential Assay Kit Abcam Cat# ab113850 Commercial assay or kit RNeasy Plus Mini Kit Qiagen Cat# 74134 Commercial assay or kit RT2 First Strand Kit Qiagen Cat# 330404 Commercial assay or kit RT2 Profiler PCR Human Apoptosis Array Qiagen Cat# PAHS-012Z Continued on next page Greenlee et al. eLife 2021;10:e67750. .. DOI: https://doi.org/10.7554/eLife.67750 19 of 30 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm CFX Maestro Software Bio-Rad RRID:SCR_018064 Software, algorithm GeneGlobe Data Analysis Center Qiagen RRID:SCR_021211 Commercial assay or kit Gene Knockout Kit v2 – Human CASP10 with Cas9 2NLS Nuclease Synthego sgRNA:Cas9 (90 pmol:10 pmol) Commercial assay or kit Vybrant Alexa Fluor 488 Lipid Raft Labeling Kit Invitrogen Cat# V34403 Commercial assay or kit Vybrant Alexa Fluor 555 Lipid Raft Labeling Kit Invitrogen Cat# V34404 Antibody Mouse anti-human CD261 (DR4) Monoclonal Antibody (clone DJR1) Invitrogen Cat# 14-6644-82 RRID:AB_468188 (1:50 IF) Antibody Mouse anti-human CD262 (DR5) Monoclonal Antibody (clone DJR2-4) Invitrogen Cat# 14-9908-82 RRID:AB_468592 (1:50 IF) Antibody Alexa Fluor 555 goat anti-mouse IgG (H+L) Invitrogen Cat# A28180 RRID:AB_2536164 (1:1000 IF) Other DAPI Invitrogen Cat# D1306 RRID:AB_2629482 (1 mg/ml) Software, algorithm Fiji – ImageJ FIJI RRID:SCR_002285 JaCOP plugin Antibody Human TruStain FcX BioLegend Cat# 422301 RRID:AB_2818986 Antibody PE mouse antihuman CD261 (DR4) (clone DJR1) BioLegend Cat# 307206 RRID:AB_2287472 (5 ml per sample, FC) Antibody PE mouse antihuman CD262 (DR5) (clone DJR2-4) BioLegend Cat# 307406 RRID:AB_2204926 (5 ml per sample, FC) Antibody PE mouse antihuman TRAILR3 (DcR1) (clone DJR3) BioLegend Cat# 307006 RRID:AB_2205089 (5 ml per sample, FC) Antibody PE mouse antihuman TRAILR4 (DcR2) (clone 104918) BioLegend Cat# FAB633P RRID:AB_2205217 (5 ml per sample, FC) Antibody PE Mouse IgG1 k Isotype Control (clone MOPC-21) BioLegend Cat# 400114 RRID:AB_326435 (5 ml per sample, FC) Antibody FITC mouse anti-human DR4 (clone DR-4-02) Thermo Fisher Scientific Cat# MA1-19757 RRID:AB_1955203 (5 ml per sample, FC) Chemical compound, drug Resveratrol Sigma-Aldrich Cat# R5010-100MG RRID:AB_309682 CAS: 501-36-0 Continued on next page Greenlee et al. eLife 2021;10:e67750.
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